10 results found for: “brdu”.

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Bromodeoxyuridine

(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used...

Last Update: 2023-08-18T21:53:04Z Word Count : 1078

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Androgen

lack of external male genitalia. Neural injections of Bromodeoxyuridine (BrdU) were applied to males of both groups to test for neurogenesis. Analysis...

Last Update: 2024-03-23T22:29:08Z Word Count : 3165

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Hoechst stain

cell-permeability than H 33258. Cells can integrate BrdU in newly synthesized DNA as a substitute for thymidine. When BrdU is integrated into DNA, it is supposed that...

Last Update: 2024-01-05T21:31:32Z Word Count : 1285

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S phase index

the capacity of tumor cells to proliferate. It is defined as the number of BrdU-incorporating cells relative to the volume of DNA staining determined from...

Last Update: 2018-01-12T00:05:15Z Word Count : 106

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Thymidine

be measured this way in lymphoproliferative disorders. Bromodeoxyuridine (BrdU) is another thymidine analog that is often used for the detection of proliferating...

Last Update: 2024-02-16T04:54:33Z Word Count : 778

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Republika Srpska

November 2017. Retrieved 29 August 2011. "Pobjednik Grmečke koride na Popovića brdu prošlogodišnji - bik Medonja". Sve o Srpskoj (in Serbian (Cyrillic script))...

Last Update: 2024-03-27T14:29:32Z Word Count : 9426

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Čukarički Stadium

Čukarički Stadium, also known as Stadion na Banovom brdu (English: Banovo Brdo Stadium) is a multi-purpose stadium in Belgrade, Serbia. It is currently...

Last Update: 2023-09-13T18:49:03Z Word Count : 96

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Local lymph node assay

specific for BrdU). The test material is applied to the ears of mice. Optionally, a tracer substance such as 3H-Methyl-thymidine or BrdU is injected intraperitoneally...

Last Update: 2022-05-23T16:14:43Z Word Count : 441

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Breg pri Golem Brdu

Breg pri Golem Brdu (pronounced [ˈbɾeːk pɾi ˈɡɔːlɛm ˈbəɾdu]) is a small settlement south of Golo Brdo in the Municipality of Brda in the Littoral region...

Last Update: 2022-10-09T03:01:40Z Word Count : 80

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Novak Djokovic

Mirjana Despot (16 October 2015). "Banjaluka dobila Novak Café na Banj brdu". PressRS.ba. Archived from the original on 9 August 2016. Retrieved 12 June...

Last Update: 2024-03-27T20:31:27Z Word Count : 38766

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Main result

Bromodeoxyuridine

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used to study cell proliferation in living tissues and has been studied as a radiosensitizer and diagnostic tool in people with cancer.During the S phase of the cell cycle (when DNA replication occurs), BrdU can be incorporated in place of thymidine in newly synthesized DNA molecules of dividing cells. Cells that have recently performed DNA replication or DNA repair can be detected with antibodies specific for BrdU using techniques such as immunohistochemistry or immunofluorescence. BrdU-labelled cells in humans can be detected up to two years after BrdU infusion.Because BrdU can replace thymidine during DNA replication, it can cause mutations, and its use is therefore potentially a health hazard. However, because it is neither radioactive nor myelotoxic at labeling concentrations, it is widely preferred for in vivo studies of cancer cell proliferation. However, at radiosensitizing concentrations, BrdU becomes myelosuppressive, thus limiting its use for radiosensitizing.BrdU differs from thymidine in that BrdU substitutes a bromine atom for thymidine's CH3 group. The Br substitution can be used in X-ray diffraction experiments in crystals containing either DNA or RNA. The Br atom acts as an anomalous scatterer and its larger size will affect the crystal's X-ray diffraction enough to detect isomorphous differences as well.Bromodeoxyuridine releases gene silencing caused by DNA methylation.BrdU can also be used to identify microorganisms that respond to specific carbon substrates in aquatic and soil environments. A carbon substrate added to the incubations of environmental samples will cause the growth of microorganisms that can utilize that substrate. These microorganisms will then incorporate BrdU into their DNA as they grow. Community DNA can then be isolated and BrdU-labeled DNA purified using an immunocapture technique. Subsequent sequencing of the labeled DNA can then be used to identify the microbial taxa that participated in the degradation of the added carbon source. However, it is not certain whether all microbes present in an environmental sample can incorporate BrdU into their biomass during de novo DNA synthesis. Therefore, a group of microorganisms may respond to a carbon source but go undetected using this technique. Additionally, this technique is biased towards identifying microorganisms with A- and T-rich genomes. DNA with BrdU transcribes as usual DNA, with guanine included in RNA as a complement to BrdU.


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