10 results found for: “proteinase_k”.

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Proteinase K

molecular biology, Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium...

Last Update: 2023-11-07T16:42:20Z Word Count : 1034

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Prion

to relate to PrP structure or function. PrPC is readily digested by proteinase K and can be liberated from the cell surface in vitro by the enzyme phosphoinositide...

Last Update: 2024-03-26T17:17:13Z Word Count : 10636

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Protein K (porin)

Proteinase K has a catalytic triad of Ser 224, His 69, and Asp 39. Two peptide chains recognize substrates, 99-104 and 132–136. There is also a cysteine...

Last Update: 2023-01-26T01:56:53Z Word Count : 269

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Staphylococcus haemolyticus

carbohydrates, proteins, and extracellular DNA. Detachment assays with NaIO4, proteinase K, or DNase result in 38%, 98%, and 100% detachment, respectively. The...

Last Update: 2023-11-28T19:27:54Z Word Count : 2402

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Proteolysis

activity or removal of unwanted proteins. For example, proteinase K, a broad-spectrum proteinase stable in urea and SDS, is often used in the preparation...

Last Update: 2023-11-07T15:44:57Z Word Count : 4225

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Cross-linking immunoprecipitation

separated from free RNA using gel electrophoresis and membrane transfer. Proteinase K digestion is then performed in order to remove protein from the crosslinked...

Last Update: 2024-02-22T11:50:47Z Word Count : 4888

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Complementary DNA

RNA unusable. To remove DNA and proteins, enzymes such as DNase and Proteinase K are used for degradation. Importantly, RNA integrity is maintained by...

Last Update: 2024-02-18T19:34:12Z Word Count : 2275

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Aspergillopepsin I

Aspergillus acid proteinase, Aspergillus aspartic proteinase, Aspergillus awamori acid proteinase, Aspergillus carboxyl proteinase, carboxyl proteinase, Aspergillus...

Last Update: 2023-08-26T13:15:44Z Word Count : 499

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In situ hybridization

fluorescence or brightfield microscope. permeabilization of cells with proteinase K to open cell membranes (around 25 minutes, not needed for tissue sections...

Last Update: 2024-03-19T17:22:13Z Word Count : 1318

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DNA extraction

used to break open cells and release the DNA. Protease enzymes, such as Proteinase K, which are used to digest proteins that may be binding to the DNA. Phenol...

Last Update: 2024-03-09T15:17:10Z Word Count : 3168

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Main result

Proteinase K

In molecular biology, Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium album proteinase K) is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Parengyodontium album (formerly Engyodontium album or Tritirachium album). Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8 (subtilisin). The molecular weight of Proteinase K is 28,900 daltons (28.9 kDa).


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