Base calling

Base calling is the process of assigning nucleobases to chromatogram peaks, light intensity signals, or electrical current changes resulting from nucleotides passing through a nanopore. One computer program for accomplishing this job is Phred, which is a widely used base calling software program by both academic and commercial DNA sequencing laboratories because of its high base calling accuracy.^[1]

Base callers for Nanopore sequencing use neural networks trained on current signals obtained from accurate sequencing data. ^[2]