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EcoRV
| Restriction endonuclease EcoRV | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 EcoRV crystal structure complexed with double-stranded DNA | |||||||||
| Identifiers | |||||||||
| Symbol | Endonuc-EcoRV | ||||||||
| Pfam | PF09233 | ||||||||
| InterPro | IPR015314 | ||||||||
| SCOP2 | 1sx5 / SCOPe / SUPFAM | ||||||||
| |||||||||
EcoRV (pronounced "eco R five") is a type II restriction endonuclease isolated from certain strains of Escherichia coli. It has the alternative name Eco32I.
In molecular biology, it is a commonly used restriction enzyme. It creates blunt ends. The enzyme recognizes the palindromic 6-base DNA sequence 5'-GAT|ATC-3' and makes a blunt end at the vertical line.[1] The complementary sequence is then 3'-CTA|TAG-5'. The ends are blunt and can be ligated into a blunt cloning site easily but with lower efficiency than sticky ends.
- ^ Schildkraut I, Banner CD, Rhodes CS, Parekh S (1984). "The cleavage site for the restriction endonuclease EcoRV is 5'-GAT/ATC". Gene. 27 (3): 327–329. doi:10.1016/0378-1119(84)90078-7. PMID 6329909.
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