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ISAS (Ictal-Interictal SPECT Analysis by SPM) is an objective tool for analyzing ictal vs. interictal SPECT scans. The goal of ictal SPECT is to localize the region of seizure onset for epilepsy surgery planning. ISAS was introduced and validated in two recent studies (Chang et al., 2002; McNally et al., 2005). This site is a technical supplement to (McNally et al., 2005), which should enable ISAS to be implemented at any center for further study and analysis.