Optogenetics

Optogenetics is a biological technique to control the activity of neurons or other cell types with light. This is achieved by expression of light-sensitive ion channels, pumps or enzymes specifically in the target cells. On the level of individual cells, light-activated enzymes and transcription factors allow precise control of biochemical signaling pathways.^[1] In systems neuroscience, the ability to control the activity of a genetically defined set of neurons has been used to understand their contribution to decision making,^[2] learning,^[3] fear memory,^[4] mating,^[5] addiction,^[6] feeding,^[7] and locomotion.^[8] In a first medical application of optogenetic technology, vision was partially restored in a blind patient with Retinitis pigmentosa.^[9]

Optogenetic techniques have also been introduced to map the functional connectivity of the brain.^[10][11] By altering the activity of genetically labelled neurons with light and by using imaging and electrophysiology techniques to record the activity of other cells, researchers can identify the statistical dependencies between cells and brain regions.^[12][13]

In a broader sense, the field of optogenetics also includes methods to record cellular activity with genetically encoded indicators.

In 2010, optogenetics was chosen as the "Method of the Year" across all fields of science and engineering by the interdisciplinary research journal Nature Methods.^[14] In the same year an article on "Breakthroughs of the Decade" in the academic research journal Science highlighted optogenetics.^[15][16][17]