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A pupa is the life stage of some insects undergoing transformation between immature and mature stages.
Pupa may also refer to:
- Pupa (gastropod), a genus of small sea snails
- Pupa (Hasidic dynasty), in Judaism
- Pupa (manga), a horror manga series
- "Pupa", song by Aidan from This Is Aidan, 2023
- Alba Encarnación (1956–2012), nicknamed "Pupa", a Puerto Rican community activist
- Metal Gear PUPA, an AI weapon and minor antagonist in the stealth video game Metal Gear Solid: Peace Walker