Sf9 (cells)

Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue.^[1] They can be grown in the absence of serum, and can be cultured attached or in suspension.^[2]

^ Vaughn JL; Goodwin RH; Tompkins GJ; McCawley P (1977). "The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae)". In Vitro. 13 (4): 213–217. doi:10.1007/BF02615077. PMID 68913. S2CID 13529129.
^ Invitrogen (14 Mar 2017). "Cell Lines". Growth and Maintenance of Insect cell lines: User Guide (PDF). Rev. Date - 8 March 2010. Invitrogen. Manual part no. 25-0127, MAN0000030. Retrieved 12 May 2017.

[1] Vaughn JL; Goodwin RH; Tompkins GJ; McCawley P (1977). "The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae)". In Vitro. 13 (4): 213–217. doi:10.1007/BF02615077. PMID 68913. S2CID 13529129.

[2] Invitrogen (14 Mar 2017). "Cell Lines". Growth and Maintenance of Insect cell lines: User Guide (PDF). Rev. Date - 8 March 2010. Invitrogen. Manual part no. 25-0127, MAN0000030. Retrieved 12 May 2017.

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