U.S. Route 301

U.S. Route 301
US 301 highlighted in red
Route information
Auxiliary route of US 1
Length	1,099 mi (1,769 km)
Existed	1932–present
Major junctions
South end	US 41 in Sarasota, FL
Major intersections	I-75 near Tampa, FL I-10 in Baldwin, FL I-16 near Register, GA I-26 near Orangeburg, SC I-74 / US 74 near McDonald, NC US 64 in Rocky Mount, NC I-64 in Richmond, VA I-95 (numerous locations) US 50 near Bowie, MD I-97 near Annapolis, MD
North end	DE 1 in Biddles Corner, DE
Location
Country	United States
States	Florida, Georgia, South Carolina, North Carolina, Virginia, Maryland, Delaware
Highway system
United States Numbered Highway System List Special Divided

U.S. Route 301 (US 301) is a spur of U.S. Route 1 running through the South Atlantic states. It runs 1,099 miles (1,769 km) from Biddles Corner, Delaware, at Delaware Route 1 to Sarasota, Florida, at U.S. Route 41. It passes through the states of Delaware, Maryland, Virginia, North Carolina, South Carolina, Georgia, and Florida. It goes through the cities of Middletown, Delaware; Annapolis, Maryland; Richmond and Petersburg, Virginia; Rocky Mount, Wilson, and Fayetteville, North Carolina; Florence, South Carolina; Statesboro and Jesup, Georgia; and Jacksonville, Ocala, Zephyrhills, and Sarasota, Florida.

US 301 is paralleled by Interstate 95 for much of its routing through The Carolinas and Virginia including short concurrencies in Santee, South Carolina, and Lumberton, North Carolina. It has a number of other concurrencies along its route.

As originally commissioned, US 301's northern terminus was at US 1 in Petersburg, Virginia. The original southern end was at a junction with then-US 17 between Florence and Marion, South Carolina.