Van Cleef is a Dutch surname. Notable people with the surname include:
- Jan Van Cleef (1646-1716), Flemish painter
- Joos van Cleve (also spelled Jos van Cleef) (1485-1540), painter from Cleves
- Cornelius van Cleve (1520-1567), painter and son of Joos
- Lee Van Cleef (1925-1989), American film actor who primarily starred in westerns
- June Van Cleef (born 1941), American photographer
- Nathan van Cleef, one of the villains of Shanghai Noon (modeled after Lee Van Cleef)